Tracers / [68Ga]Ga-FAPI-46

[68Ga]Ga-FAPI-46

Also written as [68Ga]FAPI-46; [68Ga]Ga-FAPI-46; [Ga-68]FAPI-46; [Ga-68]Ga-FAPI-46; FAPI-46; Ga-68 FAPI-46; Ga-68-FAPI-46
Radionuclide
Ga-68 t½ 67.7 min
Modality
PET
Production route
Generator / Cyclotron liquid target
Stage
Clinical research
Syntheses indexed
77

What it is

Compound class
Small molecule (quinoline)
Target / mechanism
Fibroblast activation protein (FAP) PMID 30850501 / DOI 10.2967/jnumed.118.224469
Clinical application
Broad oncology imaging (many carcinomas); also non-oncologic fibrosis and inflammation PMID 36594838 / DOI 10.1148/radiol.220749
Theranostic pair
<a href="/tracers/lu177-fapi46/">lu177-fapi46</a>

Regulatory status

FDA
No Drugs@FDA / openFDA drug registry — no approved application found; search: https://www.accessdata.fda.gov/scripts/cder/daf/index.cfm?event=BasicSearch.process&searchTerm=FAPI-46
EMA (centralised)
Other approvals
Brand names

Clinical use

Typical injected activity
80-200 MBq (most common across FAPI PET studies) PMID 38052711 / DOI 10.1053/j.semnuclmed.2023.11.003
Uptake time
~60 min post-injection (imaging feasible earlier, <30 min, for FAPI-46) PMID 38052711 / DOI 10.1053/j.semnuclmed.2023.11.003

Reported in the literature. Not a dosing recommendation.

Published syntheses

ModuleCassettePurificationYield %RCP %Precursor / supplierSource
iMiDEV
PMB-Alcen
vendor cassetteSPE44 ± 5
yes
97.7 ± 0.5% (HPLC)FAPI-46
MedChemTronica, Sollentuna, Sweden
38091157
2023 EJNMMI Radiopharm Chem
Microfluidic-based production of [68Ga]Ga-FAPI-46 and [68Ga]Ga-DOTA-TOC using the cassette-based iMiDEV™ microfluidic radiosynthesizer — The radiochemical yielddc (RCYdc) of [68Ga]Ga-FAPI-46 was 44 ± 5% (n = 3)
iMiDEV
PMB-Alcen
vendor cassetteSPE42 ± 6≥ 95%FAPI-46
MedChemTronica, Sollentuna, Sweden
38091157
2023 EJNMMI Radiopharm Chem
it was repeated at Nancyclotep (radiochemistry laboratory) using the same experimental settings as the validation runs — The pulse flow approach allowed us to obtain [68Ga]Ga-FAPI-46 with 42 ± 6% (n = 3) radiochemical yield and high radiochemical purity (≥ 95%).
EasyOne
Trasis
vendor cassetteSPE91 ± 5%
yes
>99.7%FAPI-46
SOFIE
41701433
2026 EJNMMI Radiopharm Chem
a standard EasyOne module — The corrected yield of the labelled products was 91 ± 5% (72 ± 5% non-decay corrected).
Sofie Biosciences
SOFIE
40272498
2025 Eur J Nucl Med Mol Imaging
miniAllinOne
Trasis
vendor cassetteSPE89.4 ± 0.499.2 ± 0.1FAPI-46 precursor
SOFIE
39076612
2024 Front Chem
an automated radiosynthesis of [68Ga]Ga-FAPI-46 on the Trasis miniAllinOne synthesizer — Radiochemical yield (%)n.r89.5 ± 2.691.9 ± 1.589.4 ± 0.4
miniAllinOne
Trasis
vendor cassetteSPE89.5 ± 2.699.6 ± 0.2FAPI-46 precursor
SOFIE
39076612
2024 Front Chem
on the Trasis miniAllinOne (miniAIO) module — Radiochemical yield (%)n.r89.5 ± 2.691.9 ± 1.589.4 ± 0.4
miniAllinOne
Trasis
vendor cassetteSPE91.9 ± 1.599.2FAPI-46 precursor
SOFIE
39076612
2024 Front Chem
on the miniAIO — Radiochemical yield (%)n.r89.5 ± 2.691.9 ± 1.589.4 ± 0.4
EasyOne
Trasis
vendor cassetteSPE99.3 ± 0.2FAPI-46 precursor
SOFIE
39076612
2024 Front Chem
The radiosynthesis of [68Ga]Ga-FAPI-46 was performed on the Trasis EasyOne and miniAllinOne synthesizer — Radiochemical yield (%)n.r
FASTlab 2
GE HealthCare
custom manifoldSPE95.31, 95.24, 96.40DOTA-FAPI-46
SOFIE
37843670
2023 EJNMMI Radiopharm Chem
The GE FASTlab 2 automated radiosynthesis module was used with the developer control software.
Scintomics GRP / GRP-3V
Scintomics
vendor cassetteSPE72.6 ± 4.9%
yes
97.6 ± 0.3%FAPI-46 precursor
SOFIE
37631053
2023 Pharmaceuticals (Basel)
The radiolabelling was carried out on a Scintomics GRP module (Fürstenfeldbruck, Germany). — the average decay-corrected radiochemical yield was 72.6 ± 4.9%.
EasyOne
Trasis
vendor cassetteSPE92.45%
yes
>99.4%FAPI-46
SOFIE
35904684
2022 EJNMMI Radiopharm Chem
employing a Trasis EasyOne synthesizer — Trasis GalliaPharm Acetate 99.4% 92.45%
Synthra GaSy
Synthra
custom manifoldSPE92.86%
yes
>99.8%FAPI-46
SOFIE
35904684
2022 EJNMMI Radiopharm Chem
the GaSy module from Synthra with a customized synthesis template and a customized single use cassette — Synthra GalliaPharm HEPES 99.8% 92.86%
Scintomics GallElut
Scintomics
fixed tubingSPE93.3%
yes
>99.7%FAPI-46
SOFIE
35904684
2022 EJNMMI Radiopharm Chem
the automatic synthesis of [68Ga]Ga-FAPI-46 was established on a GallElut synthesis module from Scintomics with fixed tubing — Scintomics iThemba HEPES 99.7% 93.3%
AllinOne (AiO)
Trasis
vendor cassetteSPE66.0 ± 7.6%
yes
>98%FAPI-46
SOFIE
35163938
2022 Molecules
Trasis AllinOne (AiO)™ (Trasis, Ans, Belgium) — RCY (decay corrected at the beginning of reaction): 66.0 ± 7.6 % (n = 10)
Modular-Lab PharmTracer
Eckert & Ziegler
vendor cassetteSPE57%>95%FAPI-46
SOFIE
34439784
2021 Biomolecules
Automated radiosynthesis was performed on an Eckert & Ziegler Modular-Lab PharmTracer synthesis module using the Modular-Lab software (Eckert & Ziegler). — RCYs of 57% and 64% were obtained for [68Ga]Ga-FAPI-46 and [68Ga]Ga-DOTATOC, respectively.
Modular-Lab PharmTracer
Eckert & Ziegler
custom manifoldnone95.2 ± 1.499.7%FAPI-46
ABX
33331982
2020 EJNMMI Radiopharm Chem
Modular Lab PharmTracer (MLPT) — 5096.0 ± 0.62.1 ± 0.80.5 ± 0.10.5 ± 0.20.9 ± 0.295.2 ± 1.4
Modular-Lab EAZY
Eckert & Ziegler
vendor cassettenone89.7 ± 6.799.7%FAPI-46
ABX
33331982
2020 EJNMMI Radiopharm Chem
Modular Lab eazy (ML eazy) — 5089.7 ± 6.76.1 ± 5.50.0 ± 0.00.5 ± 0.13.7 ± 1.4
Modular-Lab PharmTracer
Eckert & Ziegler
HPLC+SPE87.9 ± 1.3%98.34 ± 0.82FAPi-46
MedChemExpress
42075850
2026 Pharmaceuticals (Basel)
radiolabeling was performed using an automated synthesis module (Lab PharmTracer, Eckert & Ziegler, Berlin, Germany) — Automated synthesis of [68Ga]Ga-FAPi-46 achieved a high radiochemical yield (87.9 ± 1.3%)
Synthera
IBA
vendor cassetteSPE90.53%
yes
98.94 ± 0.72%FAPI-46
ABX
37894781
2023 Int J Mol Sci
IBA Synthera® Extension module — with values of 90.53% and 98.94% using radio-High-Performance Liquid Chromatography (radio-HPLC) respectively
FAPI-46 precursor
U. Haberkorn, Heidelberg, Germany
34957527
2022 Eur J Nucl Med Mol Imaging
41824221
2026 EJNMMI Res
Manual synthesis42488187
2026 Iran J Pharm Res
[68Ga]Ga-FAPI-46 (100 μL, 3.7 MBq; reference group)
42478500
2026 Mol Pharm
42318157
2026 Oncol Rev
FAPI-4642242868
2026 J Nucl Med
>95%DOTA-FAPI-4642230823
2026 Sci Rep
FAPI-46 precursor
SOFIE
42086826
2026 Eur J Nucl Med Mol Imaging
Modular-Lab PharmTracer
Eckert & Ziegler
HPLC+SPE87.9 ± 1.3%98.34 ± 0.82FAPi-46
MedChemExpress
42075850
2026 Pharmaceuticals (Basel)
radiolabeling was performed using an automated synthesis module (Lab PharmTracer, Eckert & Ziegler, Berlin, Germany) — achieved a high radiochemical yield (87.9 ± 1.3%)
Manual synthesis97.5%FAPI-4642010411
2026 J Nanobiotechnology
a bleomycin-induced lung fibrosis model was used. 7 days after bleomycin instillation, mice were intravenously injected with each treatment — The labeling efficiency of [68Ga]Ga-FAPI-46 reached 97.5% (Fig. S4D).
Modular-Lab PharmTracer
Eckert & Ziegler
vendor cassetteapproximately 90%
yes
>93%FAPI-46 GMP-grade precursor
SOFIE
42002985
2026 Clin Physiol Funct Imaging
an automated Modular‐Lab PharmTracer synthesis module (Eckert & Ziegler) — yielded approximately 90% [⁶⁸Ga]Ga‐FAPI‐46 (decay‐corrected)
41984469
2026 JAMA Cardiol
41871918
2026 RMD Open
41851386
2026 Sci Rep
SPE95–98%FAPI-4641762411
2026 EJNMMI Radiopharm Chem
EasyOne
Trasis
vendor cassetteSPE91
yes
>99.7%FAPI-46 peptide
SOFIE
41701433
2026 EJNMMI Radiopharm Chem
a standard EasyOne module — The corrected yield of the labelled products was 91 ± 5% (72 ± 5% non-decay corrected)
41599688
2026 Pharmaceuticals (Basel)
41540241
2026 Eur J Nucl Med Mol Imaging
Modular-Lab Standard
Eckert & Ziegler
vendor cassetteSPE87.69±5.9697.17±0.68FAPi-4641370506
2025 Einstein (Sao Paulo)
A Modular-Lab Standard synthesis module (Eckert & Ziegler, Berlin, Germany) was successfully installed and implemented in November 2013 — Labeling Yield (%)89.03±3.7894.31±6.1881.31±0.3187.69±5.96
41314671
2025 RMD Open
Modular-Lab EAZY
Eckert & Ziegler
vendor cassetteSPE>95%FAPI-46
ABX
41155591
2025 Pharmaceuticals (Basel)
The radiolabeling of [68Ga]Ga-FAPI-46 is performed using Modular-Lab Eazy Synthesizer (Eckert & Ziegler Radiopharma GmbH, Berlin, Germany) in Laboratory 1
miniAllinOne
Trasis
vendor cassetteSPE>95%FAPI-46
ABX
41155591
2025 Pharmaceuticals (Basel)
Mini AllinOne-MiniAIO (Trasis SA, Ans, Belgium) in Laboratory 2
41101974
2026 J Nucl Med
99.3% ± 0.2%FAPI-4640935615
2025 J Nucl Med
Manual synthesisFAPI-4640841501
2025 EJNMMI Radiopharm Chem
FAPI-46 was synthesized by following the literature
FAPI-46 precursor
GE HealthCare
40542858
2025 Eur J Nucl Med Mol Imaging
40349083
2025 Syst Rev
Sofie Biosciences
40272498
2025 Eur J Nucl Med Mol Imaging
40263181
2025 Mol Diagn Ther
40263181
2025 Mol Diagn Ther
SPE>95%FAPI-46
Nanchang Tanzhen Biological Technology Co., Ltd., China
40177850
2025 Mol Cancer Ther
39956945
2025 J Cachexia Sarcopenia Muscle
Manual synthesisSPE>95%FAPI-4639800373
2025 J Immunother Cancer
approximately 25 nmol of FAPI-46 and DOTA-2P(FAPI)2 was dissolved in 1 mL of NaAc (0.25 M aqueous solution), and 4 mL of 68GaCl3 solution (1.1 GBq, dissolved in 0.05 M HCl) was added
Manual synthesisSPE>99%FAPI-4639770488
2024 Pharmaceuticals (Basel)
During the labeling process, the generator was rinsed with 2 mL of 0.05 M HCl, and the waste solution was discarded.
39207485
2024 Eur J Nucl Med Mol Imaging
iMiDEV
PMB-Alcen
vendor cassetteSPE44 ± 5%
yes
97.7 ± 0.5% (HPLC)FAPI-46
MedChemTronica, Sollentuna, Sweden
38091157
2023 EJNMMI Radiopharm Chem
Microfluidic cassette-based iMiDEV™ radiosynthesizer — The radiochemical yielddc (RCYdc) of [68Ga]Ga-FAPI-46 was 44 ± 5% (n = 3)
Manual synthesisSPE>90%>95%FAPI-4637321827
2023 J Nucl Med
For 68Ga labeling, approximately 25.4 nmol of FAPI-46, DOTA-2P(FAPI)2, or DOTA-4P(FAPI)4 were dissolved in 1 mL of NaAc (0.25 M in water) and added to 4 mL of 68GaCl3 solution (1.3 GBq in 0.05 M HCl). — 68Ga, 64Cu, and 177Lu were labeled in more than 90% yield with radiochemical purity of more than 95%.
SPEmore than 95%FAPI-4634556528
2022 J Nucl Med
SPEFAPI-4641698532
2026 Ann Pharm Fr
The radiosynthesis of [68Ga]Ga-FAPI-46 was performed using a Trasis Mini AIO automated system.
SPE89.8 ± 4.8%
yes
38856210
2024 J Vis Exp
using the iPHASE MultiSyn synthesizer for clinical applications — The product was synthesized in 89.8 ± 4.8% decay corrected yield (n = 6) over 25 min.
HPLC91.1 ± 3.4 %
yes
99.8 ± 0.3 %FAPI-46 precursor39999572
2025 Nucl Med Biol
[68Ga]Ga-FAPI-46 production on a Modular-Lab PharmTracer platform for clinical application — resulted in a decay-corrected yield of 91.1 ± 3.4 % and radiochemical purity of 99.8 ± 0.3 %
70.63% ± 2.85%above 99%38145430
2024 Ann Nucl Med
full-automatic cassette-based synthesis module system Mortenon M1 for synthesis of radiopharmaceuticals — [68Ga]-FAPI-46, 70.63% ± 2.85%, n = 10
88 ± 7%>99%FAPI-4635533534
2022 Nucl Med Biol
the cassette based synthesis module Trasis EASYONE — a radiochemical yield of 88 ± 7% and 56 ± 5%
40774265
2025 Lancet Oncol
FAPI-4639603609
2024 Chem Pharm Bull (Tokyo)
an automated method for preparing [68Ga]Ga-FAPI-46 was designed using a GAIA® synthesizer
42627578
2026 Ann Nucl Med
42477144
2026 Eur J Nucl Med Mol Imaging
42341206
2026 Clin Nucl Med
42253206
2026 Expert Rev Gastroenterol Hepatol
42242866
2026 J Nucl Med
42172798
2026 Bioorg Chem
42080808
2026 Clin Nucl Med
42018345
2026 Clin Exp Rheumatol
41949416
2026 Clin Nucl Med
41854637
2026 Q J Nucl Med Mol Imaging
>95%, maximum 99.1%FAPI-4641742873
2026 Nucl Med Commun
41642049
2026 Clin Nucl Med
41506801
2026 J Nucl Med

Precursor

Common precursor
FAPI-46 conjugate PMID 33331982 / DOI 10.1186/s41181-020-00112-x
Suppliers seen in the literature
FAPI-46 precursor from SOFIE Biosciences (Dulles, VA, USA) (also cited as manufactured by ABX); FASTlab cassettes/reagents from ABX (Radeberg, Germany)
Cited suppliers in indexed syntheses
ABX, GE HealthCare, MedChemExpress, MedChemTronica, Sollentuna, Nanchang Tanzhen Biological Technology Co., Ltd., SOFIE, U. Haberkorn
Typical final purification
SPE

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Quality control Derived from the published synthesis

Ph. Eur. monograph
no specific monograph
USP monograph
not verified — the USP-NF monograph index is subscription-only and no public confirmation was found
AppearancepHRCP HPLCRCP TLCHPLC UVRadionuclidic (HPGe)Half-lifeActivityEndotoxins (LAL)SterilityFilter integrityMolar activityMetal impuritiesGe-68 breakthrough
How this set was arrived at. No monograph — base set per Ph. Eur. 0125 + rule-based. Ge-68 breakthrough + metal impurities: generator Ga-68 only. RECOMMEND ADD: molar activity (saturable FAP target).

Tests listed by reference only. Monograph texts are copyrighted and are not reproduced here. How these are derived

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