Tracers / [18F]FSPG

[18F]FSPG

Also written as (S)-4-(3-[18F]fluoropropyl)-L-glutamic acid; [18F]FSPG; [F-18]FSPG; BAY 94-9392; F-18 FSPG; FSPG
Radionuclide
F-18 t½ 109.7 min
Modality
PET
Production route
Cyclotron liquid target
Stage
Clinical research
Syntheses indexed
9

What it is

Compound class
Amino acid
Target / mechanism
Cystine/glutamate antiporter (system xc-) PMID 36525163 / DOI 10.1007/s11307-022-01793-3
Clinical application
Oncology imaging; assessment of intracellular redox / oxidative-stress status PMID 36525163 / DOI 10.1007/s11307-022-01793-3
Theranostic pair

Regulatory status

FDA
No Drugs@FDA / openFDA drug registry — no approved application found; search: https://www.accessdata.fda.gov/scripts/cder/daf/index.cfm?event=BasicSearch.process&searchTerm=FSPG+glutamic+acid
EMA (centralised)
Other approvals
Brand names

Clinical use

Typical injected activity
~300 MBq (299 +/- 23 MBq dosimetry study; 285-309 MBq in NSCLC/HNSCC cohorts) PMID 23568366 / DOI 10.2967/jnumed.112.112581; PMID 38611114 / DOI 10.3390/cancers16071437
Uptake time
Static PET ~45-60 min post-injection (dosimetry series acquired 5-240 min p.i.) PMID 26785040 / DOI 10.1148/radiol.2015142000; PMID 23568366 / DOI 10.2967/jnumed.112.112581

Reported in the literature. Not a dosing recommendation.

Published syntheses

ModuleCassettePurificationYield %RCP %Precursor / supplierSource
TRACERlab FX FN
GE HealthCare
custom manifoldSPE22.56 ± 0.97%
no
96.31 ± 0.62%di-tert-butyl (2S,4S)-2-(3-((naphthalen-2 ylsulfonyl)oxy)propyl)-4 (tritylamino) pentanedioate
ABX
37461634
2023 Res Sq
GE TRACERlab™ FXFN — The average non-decay-corrected radiochemical yield was 22.56 ± 0.97% with radiochemical purity of 96.31 ± 0.62%
FASTlab
GE HealthCare
vendor cassetteSPE30.82 ± 1.60%
no
95.96 ± 0.70%di-tert-butyl (2S,4S)-2-(3-((naphthalen-2 ylsulfonyl)oxy)propyl)-4 (tritylamino) pentanedioate
ABX
37461634
2023 Res Sq
GE FASTlab™ — the average radiochemical yields was also observed to be significantly higher than from the TRACERlab counterpart, 30.82 ± 1.60% vs. 22.56 ± 0.97%, p < 0.0001
AllinOne (AiO)
Trasis
vendor cassetteSPE33.5 ± 4.9%96.8 ± 1.1%[18F]FSPG precursor (naphthyltosylate precursor)
ABX
39821860
2025 EJNMMI Radiopharm Chem
The automated synthesis module used in the study was the Trasis AIO™ (Trasis SA) — The optimised method provided [18F]FSPG in 33.5 ± 4.9% radiochemical yield in just 35 min when starting with 18–25 GBq.
FASTlab
GE HealthCare
vendor cassetteSPE38.4 ± 2.6 %
yes
>96 %[18F]FSPG precursor 1
ABX
34013395
2021 Mol Imaging Biol
The automated radiosynthesis platform used in the study was the GE FASTlab™ (GE Healthcare) — The optimized protocol produced [18F]FSPG in 38.4 ± 2.6 % radiochemical yield
TRACERlab FX FN
GE HealthCare
fixed tubingSPE22.56 ± 0.97%
no
96.31 ± 0.62%di-tert-butyl (2S,4S)-2-(3-((naphthalen-2 ylsulfonyl)oxy)propyl)-4 (tritylamino) pentanedioate
ABX
37486582
2023 EJNMMI Radiopharm Chem
GE TRACERlab™ FXFN and FASTlab™, the most widely used synthesis modules for routine clinical radiopharmaceutical production — average radiochemical yields of 22.56 ± 0.97%
FASTlab
GE HealthCare
vendor cassetteSPE30.82 ± 1.60%
no
95.96 ± 0.70%di-tert-butyl (2S,4S)-2-(3-((naphthalen-2 ylsulfonyl)oxy)propyl)-4 (tritylamino) pentanedioate
ABX
37486582
2023 EJNMMI Radiopharm Chem
FASTlab™, a cassette-based module, for easier adoption by other radiopharmaceutical manufacturing facilities — average radiochemical yields was also observed to be significantly higher than from the TRACERlab counterpart, 30.82 ± 1.60% versus 22.56 ± 0.97%
Synthra RNplus
Synthra
custom manifoldHPLC+SPE39-43
yes
95-97di-tert-butyl-(2S,4S)-2-(3-((naphthalen-2-ylsulfonyl)oxy)propyl)-4-(tritylamino)pentanedioate
ABX
36525163
2023 Mol Imaging Biol
The radiosynthesis of [18F]FSPG was performed using a customised RNPlus Research automated radiosynthesis system (Synthra GmbH, Hamburg, Germany). — [18F]FSPG was produced typically in 39–43% radiochemical yield (decay corrected) from [18F]fluoride
21750203
2011 Clin Cancer Res
SPE23568366
2013 J Nucl Med
18F-FSPG was prepared by a fully automated 2-step procedure and purified by a solid-phase extraction method.

Precursor

Common precursor
Protected FSPG precursor with an aryl sulfonate (naphthalene-/4-nitrophenyl-sulfonate) leaving group; nucleophilic [18F]fluorination then acid/base deprotection PMID 34013395 / DOI 10.1007/s11307-021-01609-w; PMID 36525163 / DOI 10.1007/s11307-022-01793-3
Suppliers seen in the literature
ABX GmbH (Radeberg, Germany)
Cited suppliers in indexed syntheses
ABX
Typical final purification
SPE

Need this precursor?We can point you to suppliers for [18F]FSPG, or handle the enquiry for you.

Get in touch

Quality control Preliminary — inferred from compound class

Ph. Eur. monograph
USP monograph
AppearancepHRCP HPLCRCP TLCHPLC cond.Radionuclidic (HPGe)Half-lifeActivityEndotoxins (LAL)SterilityFilter integrity
How this set was arrived at. base set per Ph. Eur. general monograph 0125 (rule); organic-phase radiosynthesis assumed from isotope/compound class, no synthesis data confirming it -> GC residual solvents (preliminary); conventional nucleophilic 18F-fluorination assumed from isotope/compound class, no synthesis data confirming K222 use -> Kryptofix test (preliminary); STAGE 17.4 recheck by compound class ('Amino acid'): HPLC UV -> HPLC cond. (non-aromatic glutamate analogue (4-[18F]fluoroglutamic acid) -- weak UV chromophore like glutamine/glutamic acid, not an aromatic amino acid like FET/FDOPA/FETrp/FTYR)

Tests listed by reference only. Monograph texts are copyrighted and are not reproduced here. How these are derived

Setting up quality control?We can help you scope the equipment needed to release [18F]FSPG.

Get in touch