Tracers / [18F]DPA-714

[18F]DPA-714

Also written as [18F]DPA-714; [18F]DPA714; [F-18]DPA-714; [F-18]DPA714; DPA-714
Radionuclide
F-18 t½ 109.77 min
Modality
PET
Production route
Stage
Syntheses indexed
8

What it is

Compound class
Radiolabelled pyrazolo[1,5-a]pyrimidine acetamide (second-generation TSPO ligand)
Target / mechanism
18-kDa translocator protein (TSPO), a marker of activated microglia/macrophages in neuroinflammation PMID 19223401
Clinical application
Theranostic pair

Regulatory status

FDA
EMA (centralised)
Other approvals
Brand names

Clinical use

Typical injected activity
Uptake time

Reported in the literature. Not a dosing recommendation.

Published syntheses

ModuleCassettePurificationYield %RCP %Precursor / supplierSource
TRACERlab FX FN
GE HealthCare
HPLC24 ± 3%tosylate substrate24615467
2014 Eur J Nucl Med Mol Imaging
Automated synthesis of 18F-DPA-714 was carried out using a slightly modified TRACERLab FX-FN module (GE Medical Systems). — The yield was 24 ± 3% with a specific activity of 41–107 GBq/μmol (n = 8).
Synthera
IBA
vendor cassetteHPLC+SPE24.6 ± 3.8%
no
> 99.9%N,N-Diethyl-2-(2-(4-(2-toluenesulfonyloxyethoxy)phenyl)-5,7-dimethylpyrazolo[1,5-a]pyrimidin-3-yl)acetamide
ABX
38461627
2024 Appl Radiat Isot
an automated Synthera® radiosynthesizer (Version 1, IBA Molecular) employing an Integrated Fluidic Processor™ (IFP) cassette — EOS activity yield of 24.6 ± 3.8% and a molar activity of > 18.5 TBq/mmol (500 Ci/mmol)
ELIXYS FLEX/CHEM
Sofie Biosciences
HPLC+SPE13.5 ± 3.04%
no
2-(4-(3-(2-(diethylamino)-2-oxoethyl)-5,7-dimethylpyrazolo[1,5-a]pyrimidin-2-yl)phenoxy)ethyl 4-methylbenzenesulfonate
In-house / non-commercial (various)
34871885
2022 Appl Radiat Isot
Elixys Flex/Chem (Sofie Biosciences \u2013 Dulles, VA) — [18F]DPA-714 was synthesized in a 13.5 ± 3.04% activity yield (EOS)
AllinOne (AiO)
Trasis
custom manifoldHPLC+SPE55-71
yes
100DPA-714 tosylate
PharmaSynth AS, Tartu, Estonia
34037896
2021 EJNMMI Radiopharm Chem
synthesised using an in-house designed cassette and sequence — [18F]DPA-714 was obtained with consistently high radiochemical yields of 55-71% (n=6)
iMiDEV
PMB-Alcen
vendor cassetteHPLC17 ± 6%
yes
>99%TsO-DPA-714
PharmaSynth AS, Tartu, Estonia
39692929
2024 EJNMMI Radiopharm Chem
the iMiDEV™ radiosynthesiser — RCYa–17 ± 6%10 ± 1%11%
41129212
2025 JCI Insight
27943464
2017 Chemistry
22104496
2012 Appl Radiat Isot
using a TRACERlab FX-FN synthesizer

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Quality control Preliminary — inferred from compound class

Ph. Eur. monograph
USP monograph
AppearancepHRCP HPLCRCP TLCHPLC UVRadionuclidic (HPGe)Half-lifeActivityEndotoxins (LAL)SterilityFilter integrity
How this set was arrived at. base set per Ph. Eur. general monograph 0125 (rule); organic-phase radiosynthesis assumed from isotope/compound class, no synthesis data confirming it -> GC residual solvents (preliminary); conventional nucleophilic 18F-fluorination assumed from isotope/compound class, no synthesis data confirming K222 use -> Kryptofix test (preliminary)

Tests listed by reference only. Monograph texts are copyrighted and are not reproduced here. How these are derived

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Notes

Added from reverse module search (stage 6); 8 article(s) with F-18.