Tracers / [18F]FHBG

[18F]FHBG

Also written as 9-(4-[18F]fluoro-3-hydroxymethylbutyl)guanine; [18F]FHBG; [F-18]FHBG; FHBG
Radionuclide
F-18 t½ 109.77 min
Modality
PET
Production route
Stage
Syntheses indexed
6

What it is

Compound class
Radiolabelled acycloguanosine analogue (PET reporter probe)
Target / mechanism
Herpes simplex virus type 1 thymidine kinase (HSV1-tk) / mutant HSV1-sr39tk enzyme — phosphorylation-dependent intracellular trapping PMID 17406570
Clinical application
PET reporter-gene imaging of HSV1-tk-transduced cells (gene-therapy vector tracking) PMID 17406570
Theranostic pair

Regulatory status

FDA
EMA (centralised)
Other approvals
Brand names

Clinical use

Typical injected activity
Uptake time

Reported in the literature. Not a dosing recommendation.

Published syntheses

ModuleCassettePurificationYield %RCP %Precursor / supplierSource
ELIXYS FLEX/CHEM
Sofie Biosciences
HPLC28443841
2017 Lab Chip
synthesized using the ELIXYS radiosynthesizer (Sofie Biosciences, Culver City, CA, USA)
HPLC409622172393
2012 Nucl Med Biol
automated them on an Explora General Nucleophilic double-synthesis module — competitive radiochemical yields of up to 40% in an overall synthesis time of 60±10 min
SPE8-14%
no
more than 99%tosylate precursor20032804
2010 Nucl Med Commun
A TRACERlab FXF-N synthesizer was substantially modified and adapted to the synthesis of [F]FHBG through the two-step one-pot procedure. — The decay-uncorrected radiochemical yield of [F]FHBG was 8-14% (n=10)
HPLC21.0±3.8%98±0.9%19553130
2009 Appl Radiat Isot
Tracerlab MX — Synthesis using the Tracerlab MX module showed a 21.0+/-3.8% yield of radiochemical
HPLC32.0±1.2%99.0±0.6%19553130
2009 Appl Radiat Isot
Explora RN — synthesis using the Explora RN module showed a 32.0+/-1.2% yield of radiochemical
HPLC+SPE10-15%>99%tosylate precursor14537106
2002 Mol Imaging Biol
A nuclear interface commercial synthesizer was substantially modified and adapted to the synthesis of the referred compound. — [18F]FHBG was obtained in 10-15% yield in 65 minutes including HPLC purification.

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Quality control Preliminary — inferred from compound class

Ph. Eur. monograph
USP monograph
AppearancepHRCP HPLCRCP TLCHPLC UVRadionuclidic (HPGe)Half-lifeActivityEndotoxins (LAL)SterilityFilter integrity
How this set was arrived at. base set per Ph. Eur. general monograph 0125 (rule); organic-phase radiosynthesis assumed from isotope/compound class, no synthesis data confirming it -> GC residual solvents (preliminary); conventional nucleophilic 18F-fluorination assumed from isotope/compound class, no synthesis data confirming K222 use -> Kryptofix test (preliminary)

Tests listed by reference only. Monograph texts are copyrighted and are not reproduced here. How these are derived

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Notes

Added from reverse module search (stage 6); 2 article(s) with F-18.